Coordination chemistry in molecular recognition

TL;DRAbstract

Reversible coordinative binding of Lewis basic donors to iminodiacetato (IDA) and nitrilotriacetato (NTA) metal complexes is widely used for the design of synthetic receptors binding to peptides, proteins or enzymes at physiological conditions. However, no data on the affinity of M(II)-NTA (M = Cu, Ni, Zn) to a single histidine or imidazole moiety are available. We herein report (chapter 1) the investigation of the binding affinity and thermodynamics of copper(II), nickel(II) and zinc(II) NTA complexes to histidine, imidazole and hen egg white lysozyme, bearing a single surface exposed histidine unit, by isothermal titration calorimetry at physiological conditions. Further, we describe a peptide-metal complex hybrid approach to enhance the binding affinity of Cu(II)-NTA to lysozyme. In the second part of this work (chapter 2) the rationally design of a selective peptide receptor is described. The combination of copper(II)nitrilotriacetato (NTA) complex with an ammonium-ion sensitive an

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