Rapid Resolution Liquid Chromatography Method Development and Validation for Detection of Anastrozole in Human Serum

TL;DRAbstract

Globally, breast cancer is a common cause of cancer mortality among women. Current treatment guidelines for estrogen-receptor positive breast cancer recommend the use of anastrozole as one of the alternatives to tamoxifen. To date, no rapid resolution liquid chromatography (RRLC) method for the detection of anastrozole in human serum has been developed. A simple, accurate, highly sensitive and reproducible RRLC method was developed for quantitative measurement of anastrozole in human serum with a mobile phase consisting of a mixture of acetonitrile and ammonium acetate (45:55) with 0.05% triethylamine added at a pH of 4.0 running at 0.4 mL/min. Separation was achieved using a C-18 column (3.5 µm, 150 x 4.6 mm) maintained at 30oC and the detection was monitored at 210 nm. The retention time of the drug was 9.3 min. The system suitability parameters such as capacity factor, tailing factor, resolution, and plate count as well as validation parameters such as linearity, precision and accur

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