<b>INHIBITION BY COLCHICINE OF IMMUNOREACTIVE VASOACTIVE INTESTINAL POLYPEPTIDE RELEASE FROM ANTERIOR PITUITARY CELLS IN CULTURE </b>
TL;DRAbstract
We investigated the effect of colchicine on the release of vasoactive intestinal polypeptide (VIP) from adenohypophyseal cells in culture, measuring VIP by radioimmunoassay. Additionally, prolactin (PRL) and growth hormone (GH) release was studied. Exposure of cultured pituitary cells to colchicine for 3 or 6 h caused a significant decrease in the release of immunoreactive VIP (IR-VIP) into the media, concomitantly with an increase in IR-VIP in cells. Long-term exposure to the drug (12 and 24 h) leads to a dramatic decrease in IR-VIP in cells. These findings are similar to those obtained for PRL and GH. Though the exact nature of VIP-producing adenohypophyseal cell(s) is not known, the results suggest that microtubules play an important role in the mechanism responsible for VIP release from adenohypophyseal cells in culture.
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We investigated the effect of colchicine on the release of vasoactive intestinal polypeptide (VIP) from adenohypophyseal cells in culture, measuring VIP by radioimmunoassay. Additionally, prolactin (PRL) and growth hormone (GH) release was studied. Exposure of cultured pituitary cells to colchicine for 3 or 6 h caused a significant decrease in the release of immunoreactive VIP (IR-VIP) into the media, concomitantly with an increase in IR-VIP in cells. Long-term exposure to the drug (12 and 24 h) leads to a dramatic decrease in IR-VIP in cells. These findings are similar to those obtained for PRL and GH. Though the exact nature of VIP-producing adenohypophyseal cell(s) is not known, the results suggest that microtubules play an important role in the mechanism responsible for VIP release from adenohypophyseal cells in culture.
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