Characterization of Replicative and Error-Prone DNA Polymerases

TL;DRAbstract

DNA Polymerase II (Dpo2) and DNA Polymerase III (Dpo3) are novel enzymes from Sulfolobus solfataricus, a hypterthermophilic Archeon. Other than Dpo2 and Dpo3, S. solfataricus also encodes a replicative DNA polymerase Dpo1 (or named PolB1) and a DNA-lesion bypass DNA polymerase Dpo4. Dpo1, like Dpo2, and Dpo3, is a B-family DNA polymerase while Dpo4 belongs to the Y-family. Based on our preliminary data and others’ publication, both Dpo2 and Dpo3 are characterized as having low polymerase activity and poor 3’ 5’ exonuclease activity, indicating that they are particularly inactive and error-prone DNA polymerases. Additionally, Dpo2 and Dpo3 have been shown to be involved in DNA translesion synthesis, a DNA repair process in which a polymerase, often with a larger active site, incorporates a deoxynucleotide opposite a damaged nucleotide.\n\tThe project will involve circular dichrosim spectroscopy for both Dpo2 and Dpo3. The purpose of the circular dichroism experiments is to determine wh

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