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Book Chapter10.1007/978-1-4612-0229-5_5

Using Deuterium Exchange and Mass Spectrometry to Study Protein Structure

Robert J. Anderegg-1996-01-01-Humana Press eBooks
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TL;DRAbstract

Proteins are surely among nature’s most magical molecules. Linear polymers derived from a rather small, well-defined set of amino acids, they orchestrate every cellular process with exquisite selectivity and efficiency. While the order of amino acids, the protein’s primary sequence, is important; it is really the folding of the linear chain that brings together the key functional groups to make the protein biologically active. Mass spectrometry (MS) plays an important role in protein sequence determination, and in the identification of post-translational modification [1–2], but for higher-order structural information biochemists turn to other types of spectroscopy: NMR, X-ray crystallography, circular dichroism (CD), neutron diffraction. The development of gentle ionization techniques for mass spectrometry, matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI), have tremendously expanded the impact mass spectrometry can have on protein chemistry. The maj

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Proteins are surely among nature’s most magical molecules. Linear polymers derived from a rather small, well-defined set of amino acids, they orchestrate every cellular process with exquisite selectivity and efficiency. While the order of amino acids, the protein’s primary sequence, is important; it is really the folding of the linear chain that brings together the key functional groups to make the protein biologically active. Mass spectrometry (MS) plays an important role in protein sequence determination, and in the identification of post-translational modification [1–2], but for higher-order structural information biochemists turn to other types of spectroscopy: NMR, X-ray crystallography, circular dichroism (CD), neutron diffraction. The development of gentle ionization techniques for mass spectrometry, matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI), have tremendously expanded the impact mass spectrometry can have on protein chemistry. The maj

Keywords

Mass spectrometryChemistryElectrospray ionizationProtein mass spectrometrySample preparation in mass spectrometryHydrogen–deuterium exchangeProtein foldingFolding (DSP implementation)

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