Miniaturized Systems for Cell-based Assays.

TL;DRAbstract

s3-2 Flow cytometry can immunophenotype cells and assess expression of a limited number of molecules/cell, and the Biacore and Flexchip systems can quantify protein-protein or protein-oligonucleotide interactions. However, there are currently few systems that can quantify cell function in a microfluidic system that is combined with multiplexed analyte measurements. A novel technology capable of assaying different structural and functional characteristics of cells, using blood leukocytes as a prototype cell population, will be described. This chip-based system for functional and phenotypic cell analysis that is based on surface plasmon enhanced plasmonics. Since this microchip assay system is miniaturized, small volumes of blood and minimal amounts of reagents are consumed in the analyses. In this system, multiple different leukocyte subpopulations can be individually captured by antibodies or by MHC/peptide conjugates on a single sensor chip surface. The functional activities of these

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