Temporary Brittle Bone Disease (TBBD): Analysis of an Additional 39 Cases Associated with Fetal Immobilization

TL;DRAbstract

The biologically active form of vitamin D, 1,25-dihydroxyvitamin D (1,25D), plays essential roles in calcium homeostasis, and regulates proliferation and differentiation of a variety of cells, including prostate cells.Two hydroxylation steps catalyzed by vitamin D-25-hydroxylase (25-OHase) in the liver and 25-hydroxyvitamin D (25D)-1-hydroxylase (1-OHase) in the kidneys are required for the activation of vitamin D to 1,25D.Previously, we reported that 1-OHase is also expressed in extra-renal tissues, including prostate cells, suggesting that local production of 1,25D could provide an important cell growth regulatory mechanism.Now, we present evidence that prostate cells also possess 25-OHase and are capable of metabolizing vitamin D 3 to 1,25D 3 in the immortalized PZ-HPV-7 cells derived from normal prostate tissue.Three types of 25-OHase, one mitochondrial (CYP27A1) and two microsomal (CYP3A4 and CYP2R1), have been described.Using real-time PCR we found that CYP2R1 was expressed two-f

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