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Objective To investigate the immunogenicity of vaccine strategies about human interleukin 12 associated with combined DNA (Ag85A and ESAT-6) prime-BCG boost. Methods BALB/c mice were divided into PBS negative control and 4 immunity groups: BCG group, DNA/BCG group, DNA+IL-12/BCG group and DNA/BCG+IL-12 group. All mice received three immunizations at 2-week interval. Specific IgG antibody in serum of mice was determined with indirect ELISA in 4, 6, 8 weeks respectively after final vaccination. The splenic lymphocytes of mice were separated and stimulated with PPD to measure their proliferation by flow cytometry, and to evaluate the production of interferon-γ (IFN-γ) in cell suspensions of spleen cells by ELISA. The levels of CD4(superscript +) and CD8(superscript +) T-cell on surface of spleens lymphocyte were determined by flow cytometry. Results PPD could stimulate specific IgG responses in 4 immunity groups, and the average valences of 4 groups are 1:80, 1:120, 1:160, 1:160; the sple
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Objective To investigate the immunogenicity of vaccine strategies about human interleukin 12 associated with combined DNA (Ag85A and ESAT-6) prime-BCG boost. Methods BALB/c mice were divided into PBS negative control and 4 immunity groups: BCG group, DNA/BCG group, DNA+IL-12/BCG group and DNA/BCG+IL-12 group. All mice received three immunizations at 2-week interval. Specific IgG antibody in serum of mice was determined with indirect ELISA in 4, 6, 8 weeks respectively after final vaccination. The splenic lymphocytes of mice were separated and stimulated with PPD to measure their proliferation by flow cytometry, and to evaluate the production of interferon-γ (IFN-γ) in cell suspensions of spleen cells by ELISA. The levels of CD4(superscript +) and CD8(superscript +) T-cell on surface of spleens lymphocyte were determined by flow cytometry. Results PPD could stimulate specific IgG responses in 4 immunity groups, and the average valences of 4 groups are 1:80, 1:120, 1:160, 1:160; the sple
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