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Open AccessArticle10.6084/m9.figshare.9406.v1

Growth of mitochondrial cybrid clones on galactose

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<b>Copyright information:</b>Taken from "Production of mitochondrial cybrids containing naturally occurring pathogenic mtDNA variants"Nucleic Acids Research 2006;34(13):e95-e95.Published online 2 Aug 2006PMCID:PMC1540737.© 2006 The Author(s) Cybrids were generated as detailed in Materials and Methods (). Twenty-one individual clones from donor 1 were assessed for growth in galactose. () Fifteen clones were also assessed from a second donor. Cell counts were performed and averaged from replicate plates after 3, 5 and 7 days (donor 1) and additionally after 10 days (donor 2). For brevity, cell counts are only shown for the final time point.

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<b>Copyright information:</b>Taken from "Production of mitochondrial cybrids containing naturally occurring pathogenic mtDNA variants"Nucleic Acids Research 2006;34(13):e95-e95.Published online 2 Aug 2006PMCID:PMC1540737.© 2006 The Author(s) Cybrids were generated as detailed in Materials and Methods (). Twenty-one individual clones from donor 1 were assessed for growth in galactose. () Fifteen clones were also assessed from a second donor. Cell counts were performed and averaged from replicate plates after 3, 5 and 7 days (donor 1) and additionally after 10 days (donor 2). For brevity, cell counts are only shown for the final time point.

Keywords

Mitochondrial DNABiologyGeneticsMolecular biologyGene

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