Differential expression of cytochrome P450 genes associated with benzene‐induced hematotoxicity

TL;DRAbstract

Benzene is an established human carcinogen and leukemogen. Chronic benzene exposure results in progressive depression of bone marrow function with increased risks of aplastic anemia, myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), chronic lymphocytic leukemia, and other disorders. The mechanism underlying benzene toxicity remains uncertain; an initial metabolism and bioactivation of benzene, especially by CYP2E1 in the liver, was considered a prerequisite. To identify CYP genes whose expression is aberrant in benzene poisoning, a cDNA microarray containing 32 CYP genes was used to detect differential expression of CYPs in patients with hematopoietic dysfunction of benzene exposure. Seven female shoemakers with hematological disorders (six cases of decreased peripheral white blood cells and one case of aplastic anemia) were recruited, and seven age- and gender-matched normal subjects were selected as controls. Total RNA from the two groups was prepared, followed by reverse

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