The Sheep as a Transfusion Model: Comparison of the Storage Lesion of Human and Ovine Red Blood Cell Units

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The Sheep as a Transfusion Model: Comparison of the Storage Lesion of Human and Ovine Red Blood Cell Units

John‐Paul Tung,Gabriela Šimonová,Kristen Glenister,Jennifer Fraser,Yoke Lin Fung-2013-01-01-USC Research Bank (University of the Sunshine Coast)

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TL;DRAbstract

Introduction: There is growing awareness of risks related to transfusion of stored blood products. Animal models (e.g. ovine) will bevital to investigating the underlying mechanisms. This study aimed to characterise specific aspects of the storage lesion of ovine (ov) packed red blood cell (PRBC) units and compare these to equivalent human PRBC (huPRBC) units, expanding upon our previous reports of biochemical changes and haemolysis rates. Methods: Whole blood was collected from adult Merino ewes (n=5) into blood packs containing citrate-phosphate-dextrose (CPD) and processed into leucodepleted ovPRBCs preserved in saline, adenine, glucose and mannitol (SAGM) additive solution. Equivalent huPRBCs (n=5) were provided by the Australian Red Cross Blood Service. OvPRBCs and huPRBCs were stored at 2-6 ◦ C for 42 days, and samples were collected aseptically at weekly intervals (days 1, 7, 14, 21, 28, 35 and 42). Samples underwent testing for 2,3-diphosphoglycerate (2,3- DPG) and adenosine tr

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Introduction: There is growing awareness of risks related to transfusion of stored blood products. Animal models (e.g. ovine) will bevital to investigating the underlying mechanisms. This study aimed to characterise specific aspects of the storage lesion of ovine (ov) packed red blood cell (PRBC) units and compare these to equivalent human PRBC (huPRBC) units, expanding upon our previous reports of biochemical changes and haemolysis rates. Methods: Whole blood was collected from adult Merino ewes (n=5) into blood packs containing citrate-phosphate-dextrose (CPD) and processed into leucodepleted ovPRBCs preserved in saline, adenine, glucose and mannitol (SAGM) additive solution. Equivalent huPRBCs (n=5) were provided by the Australian Red Cross Blood Service. OvPRBCs and huPRBCs were stored at 2-6 ◦ C for 42 days, and samples were collected aseptically at weekly intervals (days 1, 7, 14, 21, 28, 35 and 42). Samples underwent testing for 2,3-diphosphoglycerate (2,3- DPG) and adenosine tr

Keywords

HaemolysisWhole bloodRed blood cellAnimal scienceHemoglobinBlood preservationBlood transfusionAndrology

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