Neoplastic Transformation and Suppression of Transformation of Human Bronchial Epithelial Cells in vitro

TL;DRAbstract

We have taken the following strategy to investigate the role of oncogenes in the neoplastic transformation of human bronchial epithelial cells. First, activated protooncogenes that are associated with human lung cancer are identified. Next, these oncogenes are transferred into the progenitor epithelial cells of broncheogenic carcinoma. The preneoplastic and neoplastic cells are then selected out from the putative suppressive normal cells. The tumorigenicity of the cells containing the transfected oncogenes is then determined using the athymic nude mouse assay system. If the transfected or infected cells show increased tumorigenicity, the dysregulation in the molecular controls of growth and terminal differentiation are then investigated. The methods used to investigate tumor suppressor genes involves several different methodologies including production of somatic cell-cell hybrids with tumorigenic and non-tumorigenic cells, and analysis of mutational events in known tumor suppressor ge

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