Effects of A Gap Junction Inhibitor on Stem Cell Retention and Efficacy During Early Myocardial Ischemia

TL;DRAbstract

Bone marrow-derived mesenchymal stem cell (BM-MSC) replacement therapy is beneficial to the heart following ischemia but a significant loss of these cells within hours of administration could diminish their effect. Early coupling between BM-MSC and ischemic cardiomyocytes through gap junction (GJ) may play a detrimental role in stem cell survival and retention in the acute phase of cell therapy. We seeded 1×105 HL-1 atrial myocytes and place them in either normoxic or ischemic condition for four hours. Then, 2×104 lineage negative murine BM-MSC were seeded over the HL-1 monolayer and the cocultures were returned to incubation either in their previous conditions (normoxia, ischemia) or switched from ischemic to normoxic condition (ischemia–reperfusion) for an additional two hours after which they were lifted, labeled with fluorescent markers for dead and apoptotic cells, and subjected to flow cytometry analysis. Ischemia induced a greater proportion of dead BM-MSC over the two-hour cocu

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