Hyoscyamine and scopolamine production in transformed root cultures of Datura metel L

TL;DRAbstract

The transformed root cultures of Datura metel L (kecubung) was successfully established via Agrobacterium rhizogenes-mediated, which contained the pBI 121 plasmid harbouring the GUS and kanamycin coding genes. The transformation was biochemically confirmed with GUS assay indicated by the presence of blue spot on roots, Southern blotting and the resistance of transformed root to Kanamycin. Transformed roots were showed a typical character of transformed root, which is sensitive to exogenous IAA (auxin). Sustained root cultures appeared to produce hyoscyamine and scopolamine ten times higher than that produced in the intact plant. The ability of the transformed roots to produce hyoscyamine and scopolamine in different types of basal media used was examined. It was ubserved that Gamborg's B 5 basal medium was the best medium for root growth as well as the hyoscyamine and scopolamine production. Gamborg's B5 medium was used for subsequent studies. Among the carbon source tested, sucrose ap

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