Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production

TL;DRAbstract

We report here the enhanced hemicellulase production by a Talaromyces thermophilus strain in a fed-batch fermentation using 3.6-litre laboratory-controlled bioreactor.When grown on wheat bran, this fungus produces a wide spectrum of polysaccharide-hydrolysing enzymes, mainly endo-b-1,4-xylanase (27 U/mL), b-xylosidase (1.4 U/mL), a-L-arabinofuranosidase (1.05 U/mL) and b-D-mannosidase (0.78 U/mL).The b-xylosidase was purified and shown to hydrolyse xylobiose and short xylooligosaccharides, but it was inactive on xylan.It released xylose from xylooligosaccharides with a degree of polymerisation ranging from 2 to 5. Talaromyces thermophilus b-xylosidase activity was unaffected by high glucose or arabinose concentration (0.5 M) and retained 75 % of its original activity in the presence of 133 mM xylose.Chitosan-immobilised b-xylosidase was used in a continuous process of conversion of wheat bran hydrolysate to xylose in a packed bed reactor.Xylose production of 18.6 mg/g was reached after

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