Desarrollo y evaluación de cuatro PCRs para la detección de Salmonella en ganglios linfáticos porcinos

TL;DRAbstract

Salmonellosis is one of the major zoonoses worldwide extended, frequently
\ntransmitted by subclinically infected pigs. Thus, EU health authorities have initiated
\nsalmonellosis control programs including prevalence studies in pigs by microbiological
\nculture of lymph nodes (LN) following the ISO 6579 method. This method has some
\ndisadvantages that recommend the development of new diagnostic tools. The aim of this
\nstudy was to develop and evaluate the usefulness of new PCRs for detecting Salmonella in
\nLN pig samples, by amplifying 2 chromosomal (A and B) and 2 plasmidic (C and D) genes
\nhighly conserved in Salmonella. These PCRs were evaluated (i) in lab conditions; (ii) against
\n38 contaminants; (iii) against 132 Salmonella strains of 37 serotypes; and (iv) with 320 swine
\nLN samples. Results shown that: (i) the four PCRs were specific for Salmonella; (ii) PCR-A
\nand PCR-B had more sensitivity than PCR-C and PCR-D, unrelated to the s

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