Induction and characterization of embryogenic callus in cotyledons leaves of tabebuia roseo-alba

TL;DRAbstract

Seeds from Tabebuia roseo-alba lose viability very fast. Moreover, the seed germination rate is very low, reaching
\napproximately 40%. This study aimed at the in vitro induction of embryogenic callus. This technology allows subsequent plant
\nregeneration as an alternative for the production of T. roseo-alba seedlings. Seeds were germinated in vitro and after 20 days,
\ncotyledonary leaves, hypocotyls and root segments excised from these seedlings were used as explants. They were inoculated on MS
\nmedium supplemented with sucrose (30 g/L), agar (5.0 g/L) and different auxins. The effect of 2,4-D, picloram and NAA at
\nconcentrations 0.0, 0.5, 1.0, 2.0 and 4.0 mg/L was evaluated. For the analysis of callus with embryogenic characteristics,
\nultra-structural study by scanning electron microscopy and cytochemical test with carmine were performed. The results showed that
\nthe culture medium supplemented with 4 mg/L NAA presented induction of callus with embr

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