Methylation is Involved in Arabidopsis thaliana Recovery from Geminivirus and Prevention of Geminivirus Seed Transmission
TL;DRAbstract
While most plant viruses are RNA viruses that replicate in the cytoplasm of the host cells, members of the Geminiviridae family are unique in that they are DNA plant viruses. Geminiviruses possess either monopartite or bipartite circular single-stranded DNA (ssDNA) genomes of 2.5-3 kb, that replicate in the nucleus through double-stranded DNA (dsDNA) intermediates (Bisaro, 1996). The genome components of bipartite geminiviruses are almost entirely different in sequence except for a 200-250 bp conserved region. The geminiviruses used in my study are the bipartite Cabbage leaf curl virus (CaLCuV) of the genus Begomovirus, and the monopartite Beet curly top virus (BCTV), of the genus Curtovirus (Bisaro, 1996). Geminiviruses are typically transmitted from plant to plant via insect vectors such as the whitefly (Begomovirus) or leafhopper (Curtovirus) (Bisaro, 1996). They infect several plant species and for my studies I focused on the model plant host Arabidopsis thaliana. Geminiviruses rep
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While most plant viruses are RNA viruses that replicate in the cytoplasm of the host cells, members of the Geminiviridae family are unique in that they are DNA plant viruses. Geminiviruses possess either monopartite or bipartite circular single-stranded DNA (ssDNA) genomes of 2.5-3 kb, that replicate in the nucleus through double-stranded DNA (dsDNA) intermediates (Bisaro, 1996). The genome components of bipartite geminiviruses are almost entirely different in sequence except for a 200-250 bp conserved region. The geminiviruses used in my study are the bipartite Cabbage leaf curl virus (CaLCuV) of the genus Begomovirus, and the monopartite Beet curly top virus (BCTV), of the genus Curtovirus (Bisaro, 1996). Geminiviruses are typically transmitted from plant to plant via insect vectors such as the whitefly (Begomovirus) or leafhopper (Curtovirus) (Bisaro, 1996). They infect several plant species and for my studies I focused on the model plant host Arabidopsis thaliana. Geminiviruses rep
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