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Prevalens och genetisk karaktärisering av afrikansk svinpestvirus i vektorer och tamsvin i Uganda

Emilia Nyberg-2011-01-01-Epsilon Archive for Student Projects (University of Southampton)

TL;DRAbstract

African swine fever, ASF, was first described 1921, in Kenya. The virus has thereafter spread through many African countries as well as in Europe, Asia, South- and Central America. The virus is spread via three different cycles, of
\nwhich one is sylvatic and includes warthogs and soft ticks. The other two are between domestic pigs and the one most common one is caused by humans, who not always realize the seriousness of precautions. The infection manifests as
\nhemorrhagic fever, but can also be more or less unnoticed.
\nThe aim of this study was to use molecular tools to detect and partly genetically characterize ASFV both in soft ticks and in serum from domestic pigs in Uganda.
\n
\nComparing viruses detected in ticks with those detected in domestic pigs I also wanted to investigate possible links between the sylvatic and domestic cycle. The analysis was carried out by TaqMan PCR, with p72 as target gene, and
\nsequencing of the positive PCR products. 
\n

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African swine fever, ASF, was first described 1921, in Kenya. The virus has thereafter spread through many African countries as well as in Europe, Asia, South- and Central America. The virus is spread via three different cycles, of
\nwhich one is sylvatic and includes warthogs and soft ticks. The other two are between domestic pigs and the one most common one is caused by humans, who not always realize the seriousness of precautions. The infection manifests as
\nhemorrhagic fever, but can also be more or less unnoticed.
\nThe aim of this study was to use molecular tools to detect and partly genetically characterize ASFV both in soft ticks and in serum from domestic pigs in Uganda.
\n
\nComparing viruses detected in ticks with those detected in domestic pigs I also wanted to investigate possible links between the sylvatic and domestic cycle. The analysis was carried out by TaqMan PCR, with p72 as target gene, and
\nsequencing of the positive PCR products. 
\n

Keywords

African swine feverGenotypeBiologyAfrican swine fever virusVirologyVeterinary medicineTaqManPolymerase chain reaction

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