TL;DRAbstract
Human cytomegalovirus (HCMV) is a ubiquitous virus that infects 70-90% of the general population, primarily the immunocompromised, but has been implicated in several forms of cancer, including breast cancer. Breast cancer is the second leading cause of cancer related deaths in women in North America, usually from metastasis. Exosomes are 30-100nm vesicles produced by most cells which carry protein and RNA to cells in their microenvironment. The aim of this study is to investigate the impact of HCMV-infection of a secreted viral cytokine, cmvIL-10, on exosome production by highly metastatic breast cancer cells. MDA-MB-231 cells were cultured in vitro, and were treated with cmvIL-10. Exosomes were isolated from cell media via ultracentrifugation. A subsequent quantification colorimetric assay, quantitative polymerase chain reaction (qPCR), Western Blot and fluorescent tagging and reintroduction to untreated cells were used to determine number, content and localization of collected exosom
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Human cytomegalovirus (HCMV) is a ubiquitous virus that infects 70-90% of the general population, primarily the immunocompromised, but has been implicated in several forms of cancer, including breast cancer. Breast cancer is the second leading cause of cancer related deaths in women in North America, usually from metastasis. Exosomes are 30-100nm vesicles produced by most cells which carry protein and RNA to cells in their microenvironment. The aim of this study is to investigate the impact of HCMV-infection of a secreted viral cytokine, cmvIL-10, on exosome production by highly metastatic breast cancer cells. MDA-MB-231 cells were cultured in vitro, and were treated with cmvIL-10. Exosomes were isolated from cell media via ultracentrifugation. A subsequent quantification colorimetric assay, quantitative polymerase chain reaction (qPCR), Western Blot and fluorescent tagging and reintroduction to untreated cells were used to determine number, content and localization of collected exosom
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