Localization of alkaline phosphatase in lymphoid tissues of C57B1/6J mice during an immune response

TL;DRAbstract

The membrane-bound enzyme alkaline phosphatase has been biochemically characterized and associated with thymic lymphomas. This enzyme has been shown to be localized in the thymic dependent areas in the normal spleen of C57B1/6J mice. Since these cells undergo dedifferentiation in normal immune responses and alkaline phosphatase is associated with lymphoproliferative diseases, it was of interest to find the role of these alkaline phosphatase positive cells in normal immune responses. The C57B1/67 mice were treated with lipopolysaccharide, hydrocortisone, sheep red blood cells, and 4-ethoxymethylene-2-phenyl oxazolone. Alkaline phosphatase activity was histochemically localized in the spleen and lymph nodes. Lipopolysaccharide has been shown to be a B cell mitogen, hydrocortisone an immunosuppressant, sheep red blood cells an antigen, and 4-ethoxymethylene-2-phenyl oxazolone a T cell mitogen. Histochemical analysis revealed that following treatment by these substances, there was an incre

Chat with Paper

AI Agents for this Paper