Thrombolytic activity of Cheonggukjang kinase on the recovery of brain damage in cerebral embolic stroke model

TL;DRAbstract

Stroke has become the secondary leading cause of death and the first cause of major adult disability in the world. Cheonggukjang kinase (CGK), referring to the fibrinolytic enzyme that catalyzes the clot dissolution, was prepared from the culture of pHY3‐5 transformed Bacillus subtilis ISW 1214. Fibrinolytic activity of CGK was confirmed by fibrin clot lysis assay and platelet rich clot lysis assay. 40 hr cultured CGK has the highest plasmin‐like protease activity (44.6±1.50 mU/100 μg CGK) compared with other CGKs (24h, 14.0±2.07; 60h, 12.6±0.85; 80h, 16.3±1.36 mU/100 μg CGK). The effect of doses of CGK (0.1–1 U plasmin) on cerebral embolic stroke, injected intravenously for 10 min, 1 hr after middle cerebral artery occlusion (MCAO) showed that all CGK treated groups significantly reduced the infarct volume in a dose‐dependent manner compared to the control group, However, hemorrhage was identified in the coronal brain sections in rats injected 2 U plasmin‐like activity/kg, rt‐PA at 10

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