Nested Polymerase Chain Reaction (PCR) on Fixed Stained Slides in Comparison to Whole Blood as a Source of DNA in Southeast of Iran

TL;DRAbstract

The microscopic examination of Thick Blood Smears (TBS) remains the method of choice for the diagnosis of human malaria. Recently, alternative diagnostic methods, such as Nested PCR, have been used for the detection and identification of malaria parasites. The aim of this study was to compare the sensitivity and specificity of Nested PCR accomplished using DNA extracted from whole blood against fixed stained slides. 125 blood samples including 76(60.8%) male and 49(40.2%) female accomplished examinations. The percentage of the parasitaemia on positive samples was calculated from a total count of 200 leukocytes counted in a Giemsa stained thin blood films. The nested PCR assay carried out on DNA Extracted samples by specific primers to amplify 18ssr RNA Plasmodium gene. Of all 125 blood samples 50(40%) were positive (41(32.8%) P. vivax, 9(7.2%) P. falciparum) and 75(60%) were negative for malaria parasites using microscopy examination. Nested-PCR on whole blood specimens detected 66(52.

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