Antimicrobial resistant genotype and phenotype in nosocomial coagulase-negative staphylococci from critically ill newborns: personal experience and review

TL;DRAbstract

During a 3-year period, we screened antimicrobial resistance genotype (mecA positive or negative) in clinically significant coagulase-negative staphylococci isolates from patients residing in our neonatal intensive care unit. Using mecA gene PCR analysis and Southern blot hybridization as the gold standard, the accuracy of agar dilution MIC, E-test MIC, disk diffusion and agar screen tests in detecting oxacillin-resistance during 48 hours of incubation was evaluated. The differential in MICs susceptibilities to five non-beta-lactams selected for their relevant clinical use in our setting, was also compared with mecA status of the isolates. Finally, the performance of E-test and disk diffusion methods was compared with that of the reference agar dilution test for determining susceptibility to the five non-beta-lactams. The frequency of mecA was 48.6% among study strains. For agar dilution susceptibility testing after 48h of incubation, the specificity in detecting the 78 strains lacking

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