KLONING DAN EKSPRESI GEN ORF124 KOI HERPESVIRUS SEBAGAI KANDIDAT VAKSIN

TL;DRAbstract

Koi herpesvirus (KHV) which also known as Cyprinid herpesvirus 3 (CyHV-3), Koi herpes-like virus, and carp interstitial nephritis gill necrosis virus (CNGV), caused significant morbidity (sickness or disease) and mortality in koi and common carp (Cyprinus carpio). Mortality of KHV disease reached 80�100% in affected populations. Glycoprotein has been used for vaccine development as sub unit vaccine. The aim of this research was to clone and express membrane glycoprotein ORF124 KHV as a candidate of recombinant vaccine. ORF124 KHV gene was successfully cloned into pBSKS and sequenced. Result showed that ORF124 KHV (isolate from Indonesian) had 100 % similarity with Cyprinid herpesvirus 3 DNA strain TUMST1 (AP008984.1, isolate from Japan), 99% similarity with Koi herpesvirus strain KHV-U (DQ657948.1, isolate from USA) and Koi herpesvirus strain KHV-I (DQ177346.1, isolate from Israel). Prediction analysis of T and B cell epitopes showed that ORF124 KHV protein can be used as a vaccine c

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