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Open AccessArticle10.17221/1012-pse

Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community

Jan Kopecký,Gabriela Balíková Novotná,Markéta Ságová‐Marečková-2009-09-30-Plant Soil and Environment
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TL;DRAbstract

A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group of bacteria, actinomycetes in our case, to the universal fingerprint. The diversity of actinomycetes was evaluated directly as a percentage of the whole bacterial community by comparing the corresponding terminal fragments of the same length.

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A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group of bacteria, actinomycetes in our case, to the universal fingerprint. The diversity of actinomycetes was evaluated directly as a percentage of the whole bacterial community by comparing the corresponding terminal fragments of the same length.

Keywords

Terminal restriction fragment length polymorphismRestriction fragment length polymorphismBiologyFragment (logic)Restriction fragmentDeciduousBacterial taxonomyAmplified fragment length polymorphism

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