Micropropagation of endangered slipper orchid, Paphiopedilum rothschildianum (Rchb. F) stein

TL;DRAbstract

Paphiopedilum rothschildianum, a highly endangered slipper orchid species endemic to Mount Kinabalu, Sabah, Malaysia, is strictly protected under Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). A study was carried out to establish in vitro culture system and to develop efficient propagation strategies to prevent this species from being extinct. The effect of casein hydrolysate, peptone and tryptone peptone at concentrations of 0.5, 1.0 and 2.0 g/L on multiple shoots induction of P. rothschildianum were studied. Additions of 1.0 g/L peptone into PGR-free ½ MS has resulted in the formation of 2.9 shoots per nodal stem explants; whereas, 2.8 shoots were produced from a single shoot after 16 week of culture on PGR-free ½ MS supplemented with 2.0 g/L tryptone-peptone. The study was also conducted to investigate the effects of cytokinins, organic additives and combination of cytokinin and organic additives on multiple shoot formation of P. rothschildi

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