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Evidence for the Liberation of a Nuclease from Human Fingers

Robert W. Holley,Jean Apgar,Susan H. Merrill-1961-07-01-Journal of Biological Chemistry
50

TL;DRAbstract

TABLE III Localization of radioactivity in B,s coenzymeThe incubation conditions were identical with those described in Table I except that 0.36 wmole of ATP-8-04 (220,000 c.p.m.) was used.Radioactivity c.p.m. A. Dowex l-form&e effluent._.B. Dowex 50.Na+ effluent.C. Degradation of B by light 7900 8730 I.-Adenine nucleoside fraction.. 2-Cobamide fraction.D. Degradation of B by cyanide I-Adenine fraction.2-Cobamide fraction.6610

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TABLE III Localization of radioactivity in B,s coenzymeThe incubation conditions were identical with those described in Table I except that 0.36 wmole of ATP-8-04 (220,000 c.p.m.) was used.Radioactivity c.p.m. A. Dowex l-form&e effluent._.B. Dowex 50.Na+ effluent.C. Degradation of B by light 7900 8730 I.-Adenine nucleoside fraction.. 2-Cobamide fraction.D. Degradation of B by cyanide I-Adenine fraction.2-Cobamide fraction.6610

Keywords

Fraction (chemistry)CyanideChemistryChromatographyDegradation (telecommunications)EffluentIncubationNucleoside

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