User Settings
Open AccessArticle10.56808/2985-1130.1969

PCR-RFLP ANALYSIS IN AN OPEN READING FRAME 5 OF VARIANTS OF PRRSV ISOLATED IN THAILAND

Nusara Punprapa,Anudep Rungsipipat,Amornrat Tatsanakit,Piya Wongyanin,Roongroje Thanawongnuwech-2004-06-01-The Thai Journal of Veterinary Medicine
1PDF

TL;DRAbstract

Ten selected Thai isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were amplified in an open reading frame 5 by a reverse, transcriptase, polymerase chain reaction (RT-PCR) and cut with restriction enzymes: MluI, HincII, SacII and HaeIII for the US genotype and PstI, HaeII and ClaI for the EU genotype. None of the field isolates had a similar cutting pattern when compared to modified live virus vaccines, however, several cutting patterns were obtained from this study. The results suggested that genetic variation was present among the Thai isolates, even within the same genotype. The variations may be the result of the introduction of a new variant into the endemic area or by local evolution.

Chat with Paper

AI Agents for this Paper

Ten selected Thai isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were amplified in an open reading frame 5 by a reverse, transcriptase, polymerase chain reaction (RT-PCR) and cut with restriction enzymes: MluI, HincII, SacII and HaeIII for the US genotype and PstI, HaeII and ClaI for the EU genotype. None of the field isolates had a similar cutting pattern when compared to modified live virus vaccines, however, several cutting patterns were obtained from this study. The results suggested that genetic variation was present among the Thai isolates, even within the same genotype. The variations may be the result of the introduction of a new variant into the endemic area or by local evolution.

Keywords

HaeIIIBiologyGenotypeRestriction enzymeRestriction fragment length polymorphismVirologyPolymerase chain reactionOpen reading frame

Chat

Click to start Chat