Synergistic Effect of Ethanol and Mitomycin C on Corneal Stroma
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Purpose To investigate the combined effects of ethanol and mitomycin C (MMC) application on the corneal stroma of rabbits that underwent photorefractive keratectomy (PRK). Methods Twenty-four rabbits (24 eyes) underwent PRK to correct −9.00 diopters of myopia. Twelve eyes had ethanol application before removing the epithelium and 12 eyes had the epithelium manually removed without ethanol. Eyes in both groups had topical MMC 0.02% application for 12 seconds immediately after excimer laser ablation. Twelve rabbits were sacrificed at two time points—4 hours and 4 weeks after surgery—and immunohistochemistry was performed with TUNEL assay, alpha-smooth muscle actin (alpha-SMA), and DAPI. Results More TUNEL-positive cells were observed in the ethanol-treated group compared to the mechanical debridement group at 4 hours after surgery ( P <.01). No significant difference in alpha-SMA–positive cells was detected between the two groups at 4 weeks after sugery. However, decreased keratocyte
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Purpose To investigate the combined effects of ethanol and mitomycin C (MMC) application on the corneal stroma of rabbits that underwent photorefractive keratectomy (PRK). Methods Twenty-four rabbits (24 eyes) underwent PRK to correct −9.00 diopters of myopia. Twelve eyes had ethanol application before removing the epithelium and 12 eyes had the epithelium manually removed without ethanol. Eyes in both groups had topical MMC 0.02% application for 12 seconds immediately after excimer laser ablation. Twelve rabbits were sacrificed at two time points—4 hours and 4 weeks after surgery—and immunohistochemistry was performed with TUNEL assay, alpha-smooth muscle actin (alpha-SMA), and DAPI. Results More TUNEL-positive cells were observed in the ethanol-treated group compared to the mechanical debridement group at 4 hours after surgery ( P <.01). No significant difference in alpha-SMA–positive cells was detected between the two groups at 4 weeks after sugery. However, decreased keratocyte
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