HIV‐1‐Infected Peripheral Blood Mononuclear Cells (PBMC) Promote Neutrophil Survival and Induce GM‐CSF‐Dependent Expression of HLA‐DR and IFN‐γ‐Dependent Expression of CD64

TL;DRAbstract

We examined the ability of HIV‐1 infected PBMC to influence neutrophil function. Incubating neutrophils with supernatant of PHA‐activated PBMC that had been cultured with HIV‐1 for six days (HIV‐1 + culture supernatant) significantly increased neutrophil survival at 48 hr. Depletion of the HIV‐1 virions by centrifugation did not alter the pro‐survival activity of the HIV‐1 + culture supernatant. The HIV‐1 + culture supernatant also induced expression of HLA‐DR and CD64 by neutrophils. The induction of HLA‐DR paralleled induction of HLA‐DR expression by GM‐CSF. The HIV‐1 + culture supernatant, however, did not induce neutrophil expression of CD80, CD86, or CD40. The HIV‐1 + culture supernatant stimulated concentration‐dependent increases in neutrophil HLA‐DR and CD64 at supernatant concentrations containing 5 to 50 pg GM‐CSF/ml and 3 to 30 pg IFN‐γ/ml, respectively. Neutralizing anti‐GM‐CSF antibody inhibited HLA‐DR expression and neutralizing anti‐IFN‐γ antibody inhibited CD64 expressi

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