Caracterização morfológica, bioquimica e molecular de Vickermania Itaguaiensis N. Gen, SP (Protozoa, Kinetoplastida)

TL;DRAbstract

The Trypanosomatidae family includes parasites of a wide variety of vertebrates, invertebrates (mainly insects), plants and some species of protozoa, and after the nematodes, the highest distribution of hosts in nature. In this study, an isolate was obtained by coproculture from Leptoglossus stigma Herb, 1784 (Hemiptera, Coreidae) intestinal tract, captured in the city of Itaguai/RJ. The original isolate and clones (obtained by flow cytometry) were deposited in the Colecao de Flagelados do Laboratorio de Transmissores de Leishmaniose. One clone was characterized by several approaches in comparison with the reference species of different genus. Growth was analyzed in culture medium at 24 h intervals between 48-144 h, cell differentiation and morphometric parameters of the main evolutionary stages matches. The isoenzyme analysis was performed using the following systems: GPI, PGM, 6PGDH, HK, ACON, MPI, FUM, IDH, MDH and ACON. RAPD-PCR was performed using 6 primers, together w

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