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Article10.1097/wnr.0000000000000427

Human adipose-derived stem cells enhance the survival and neuritogenesis of auditory neurons

Philipp Schendzielorz,Kristen Rak,Jennifer Nguyen,Katrin Frölich,Agmal Scherzad,Rudolf Hagen+1 more-2015-07-23-Neuroreport
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TL;DRAbstract

To evaluate the effect of human adipose-derived stem cells (ASCs) on auditory neurons with respect to a potential application in cochlear implantation. Spiral ganglions from rats were enzymatically dissociated and cocultured with or without ASCs in a coculture system. After 24 and 48 h, inserts with ASCs were removed and the dissociated neurons were fixed and stained. Then, a cell count of all dissociated cells and neurons was performed and the length of the neurites was evaluated. The potential effect of ASCs on the direction of neurite outgrowth was evaluated in a chemotaxis assay. For this, one chamber of the assay was filled with ASCs in medium and the other chamber filled with medium only. After 48 h, the growth direction of neurite outgrowth was evaluated. Cocultivation of auditory neurons with ASCs resulted in a marked and statistically significant increase in neurite outgrowth at 24 and 48 h after plating. Moreover, the number of surviving neurons and the percentage of neurons

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To evaluate the effect of human adipose-derived stem cells (ASCs) on auditory neurons with respect to a potential application in cochlear implantation. Spiral ganglions from rats were enzymatically dissociated and cocultured with or without ASCs in a coculture system. After 24 and 48 h, inserts with ASCs were removed and the dissociated neurons were fixed and stained. Then, a cell count of all dissociated cells and neurons was performed and the length of the neurites was evaluated. The potential effect of ASCs on the direction of neurite outgrowth was evaluated in a chemotaxis assay. For this, one chamber of the assay was filled with ASCs in medium and the other chamber filled with medium only. After 48 h, the growth direction of neurite outgrowth was evaluated. Cocultivation of auditory neurons with ASCs resulted in a marked and statistically significant increase in neurite outgrowth at 24 and 48 h after plating. Moreover, the number of surviving neurons and the percentage of neurons

Keywords

NeuriteStem cellChemotaxisBiologyNeuronAdipose tissueCell biologyAnatomy

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