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Article10.1161/circ.124.suppl_21.a16777

Abstract 16777: Identification of Cardioprotective Factors Produced by Fetal Mesenchymal Stem Cells Through a Combined Transcriptomic and Proteomic Approach

Patrizia Danieli,Lennard J. M. Dekker,Lennaert Kleijn,Elisabetta Cervio,Rudol A de Boer,Theo M. Luider+1 more-2011-11-22-Circulation
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TL;DRAbstract

Background: mesenchymal stem cells of fetal origin (F-MSC) can be isolated from the amniotic membrane of human placenta. We have data showing that F-MSC exert remarkable cardioprotective and proangiogenic effects through paracrine mechanisms. However, the complete nature and scope of the soluble mediators of cardioprotection have not been investigated yet. Methods: F-MSC were isolated from human term placenta. Human dermal fibroblasts were used as control population. Total RNA and proteins were extracted from cultured F-MSC (N=10) and fibroblasts (n=10) after 48 hrs of serum deprivation. Genome-wide expression profiling was performed on RNA samples with Illumina platform. Proteins extracted from both cell populations were analyzed by liquid chromatography-Fourier transform mass spectrometry (LC-FTMS). Results: whole-transcriptome gene expression analysis revealed that 647 genes are significantly over-expressed in F-MSC vs fibroblasts. Among these genes, 131 are up-regulated more than 2

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Background: mesenchymal stem cells of fetal origin (F-MSC) can be isolated from the amniotic membrane of human placenta. We have data showing that F-MSC exert remarkable cardioprotective and proangiogenic effects through paracrine mechanisms. However, the complete nature and scope of the soluble mediators of cardioprotection have not been investigated yet. Methods: F-MSC were isolated from human term placenta. Human dermal fibroblasts were used as control population. Total RNA and proteins were extracted from cultured F-MSC (N=10) and fibroblasts (n=10) after 48 hrs of serum deprivation. Genome-wide expression profiling was performed on RNA samples with Illumina platform. Proteins extracted from both cell populations were analyzed by liquid chromatography-Fourier transform mass spectrometry (LC-FTMS). Results: whole-transcriptome gene expression analysis revealed that 647 genes are significantly over-expressed in F-MSC vs fibroblasts. Among these genes, 131 are up-regulated more than 2

Keywords

MedicineMesenchymal stem cellTranscriptomeIdentification (biology)FetusStem cellBioinformaticsComputational biology

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