The role of the metastasis promoting protein S100A4 during EMT in mammary gland epithelial cells

TL;DRAbstract

Breast cancer is one of the greatest contributors to mortality among the different cancer types in the\nfemale population of the western world each year. An increasing degree of evidence state that the\nS100A4 protein, which has been identified in several tumors of different origins and has proven to be\nassociated with a poor patient prognosis, might have an important role in a process which induces\ncarcinoma cells of the breast to gain a more motile and invasive phenotype. This process, which\nenables metastasis of carcinoma cells, is termed epithelial-to-mesenchymal transition (EMT).\nIn the current study, immortalized human mammary epithelial cells were used to investigate noncancerous\nepithelial cells ability to undergo EMT when the expression of S100A4 had been knocked\ndown by lentiviral transduction. The knock-down cells were subsequently stimulated with TGF-1 for\neight days to induce EMT.\nIn order to evaluate whether the epithelial cells had undergone EMT, established mar

Chat with Paper

AI Agents for this Paper