Ultrastructural Examination of Type I and Type II Fibers from Human Vastus Lateralis Muscle

TL;DRAbstract

It is known that human skeletal muscle has several fiber types, each with a different metabolic and physiological profile. It is not known whether there are different ultrastructural characteristics within each fiber type which may account for the different contractile properties. The purpose of this study was to examine the relationship between fiber type and ultrastructural characteristics. Needle biopsies were obtained from the right vastus lateralis of five healthy males. According to the method described by Alway et al (1980), the tissue was prepared for electron microscopy and fiber types were identified based on their myoglobin content. Three non-overlapping electron micrographs were taken from the interior of each fiber under a Philips 300 electron microscope, and 50 type I and 50 type II fibers were examined from each subject. The constituents which were studied included: myofibrillar protein, mitochondria, cytoplasm, lipid and sarcoplasmic reticulum (SR). Volume densities wer

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