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Article10.1096/fasebj.21.5.a353-c

LRAT gene promoter exhibits regulated activity, but is not responsive to retinoic acid in HEK and HepG2 cells.

Reza Zolfaghari,A. Catharine Ross-2007-04-01-The FASEB Journal
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TL;DRAbstract

Lecithin:retinol acyltransferase (LRAT) catalyzes the formation of retinyl esters and is essential for normal transport and intracellular storage of vitamin A (VA). In this study, we confirmed that liver LRAT mRNA was up‐regulated by dietary VA and exogenous retinoic acid (RA). To identify the promoter for the LRAT gene, we cloned a 3.2 kb DNA segment from rat genomic DNA, spanning 2.5 kb in the 5′ upstream to 800 bp downstream of the transcription start site (TSS) and prepared LRAT promoter‐luciferase (Luc) constructs used to transfect human kidney (HEK293T) and hepatoma (HepG2) cells. None of the promoter constructs responded to all‐trans‐RA or after cotransfection with RA receptors in either cells, but they showed significantly more activity when the cells were cotransfected with PPAR‐a and RXR‐a in the presence of 9‐cis RA and WY‐14643, a PPAR‐a ligand. The proximal region up to −268 bp from the TSS, which is conserved in mouse and rat, conferred the highest Luc activity in both ce

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Lecithin:retinol acyltransferase (LRAT) catalyzes the formation of retinyl esters and is essential for normal transport and intracellular storage of vitamin A (VA). In this study, we confirmed that liver LRAT mRNA was up‐regulated by dietary VA and exogenous retinoic acid (RA). To identify the promoter for the LRAT gene, we cloned a 3.2 kb DNA segment from rat genomic DNA, spanning 2.5 kb in the 5′ upstream to 800 bp downstream of the transcription start site (TSS) and prepared LRAT promoter‐luciferase (Luc) constructs used to transfect human kidney (HEK293T) and hepatoma (HepG2) cells. None of the promoter constructs responded to all‐trans‐RA or after cotransfection with RA receptors in either cells, but they showed significantly more activity when the cells were cotransfected with PPAR‐a and RXR‐a in the presence of 9‐cis RA and WY‐14643, a PPAR‐a ligand. The proximal region up to −268 bp from the TSS, which is conserved in mouse and rat, conferred the highest Luc activity in both ce

Keywords

Retinoic acidMolecular biologyRetinoid X receptorTransfectionTranscription (linguistics)Response elementLuciferaseCAAT box

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