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Article10.1183/13993003/erj.38.suppl_55.p2668

Accuracy of IFN-γ and IP-10 detection for diagnosis of tuberculosis in children

Irene Latorre,J San José Díez,Irene Mialdea,Neus Altet,Cristina Prat,Nuria Díez+4 more-2011-09-01-European Respiratory Journal
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TL;DRAbstract

Objective: Evaluate IP-10 detection for latent tuberculosis infection (LTBI) and active tuberculosis (TB) in children, comparing the results with IFN-γ detection. Material and methods: IFN-γ released was determined by Quantiferon-TB Gold In Tube (QFN). IP-10 was retrospectively detected in supernatants by an in-house ELISA and analyzed using preset cut offs for positive (6.4ng/ml) and indeterminate (3.5ng/ml) IP-10 test result (Ruhwald, Latorre in prep). Results: 45 pediatric patients were classified in 3 groups. Group 1: 10 children diagnosed with active TB, with a Mycobacterium tuberculosis positive culture or active clinical TB. Group 2: 15 children enrolled during LTBI screening studies, with a positive QFN. Group 3: 20 healthy control children, with a negative QFN. Sensitivities of both IFN-γ and IP-10 assays were 50% (Group 1). Specificity of IP-10 detection was 100% (Group 3). Combining both cytokines the sensitivity improved to 60%, without a compromise of the specificity. Perc

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Objective: Evaluate IP-10 detection for latent tuberculosis infection (LTBI) and active tuberculosis (TB) in children, comparing the results with IFN-γ detection. Material and methods: IFN-γ released was determined by Quantiferon-TB Gold In Tube (QFN). IP-10 was retrospectively detected in supernatants by an in-house ELISA and analyzed using preset cut offs for positive (6.4ng/ml) and indeterminate (3.5ng/ml) IP-10 test result (Ruhwald, Latorre in prep). Results: 45 pediatric patients were classified in 3 groups. Group 1: 10 children diagnosed with active TB, with a Mycobacterium tuberculosis positive culture or active clinical TB. Group 2: 15 children enrolled during LTBI screening studies, with a positive QFN. Group 3: 20 healthy control children, with a negative QFN. Sensitivities of both IFN-γ and IP-10 assays were 50% (Group 1). Specificity of IP-10 detection was 100% (Group 3). Combining both cytokines the sensitivity improved to 60%, without a compromise of the specificity. Perc

Keywords

MedicineConcordanceTuberculosisLatent tuberculosisQuantiFERONInternal medicineImmunologyGold standard (test)

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