1st activity report nat. for the study and treatment of cancer in Milan for the year 1945.

TL;DRAbstract

Objective: To investigate the effects of catechins on cell growth and apoptosis of human multiple myeloma cell line RPMI 8226 in vitro, and to explore the possible mechanism. Methods: The growth inhibitory rate and apoptosis rate of RPMI 8226 cells were detected by MTT method, Wright’s staining and flow cytometry (FCM) after treatment with different concentrations of catechins. The change of mitochondrial membrane potential induced by catechins was detected by FCM. The expression levels of Δcaspase-3 fragments and bcl-2 protein were examined by Western blotting, and the secretion level of cytokine interleukin-6 (IL-6) in RPMI 8226 cells was analyzed by enzyme-linked immunosorbent assay (ELISA). Results: The growth of RPMI 8226 cells was significantly inhibited by catechins of 129.6 or 259.1 μmol/L for 1-3 d in a dose- and time-dependent manner, and the half inhibitory concentration (IC50) was 165.7 μmol/L at 24 h. The typical morphological changes of apoptosis were observed when the RP

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