Transmission of a grapevine leafroll associated closterovirus by the scale insect Pulvinaria vitis L.

TL;DRAbstract

Transmission tests from grape to grape with the leaf roll associated closterovirus GLRaVIII were carried out using scale insects of the species Parthenolecanium corni a/id Pulvinaria vitis. Four months after inoculation feeding two out of the five vines inoculated by P. vitis began to show a slight reddening and rolling of some leaves. Hybridisation and PCR tests performed with nucleic acids extracted from the two vines showing symptoms confirmed the presence of GLRaV-III. This seems to be the first report of an experimental virus transmission by scale insects.

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