Comparative in-vitro study of killing activities and morphological changes of Cefpirome, Cefepime, Imipenem and Meropenem alone and in combination against gram negative bacteria

TL;DRAbstract

The beta-lactam antibiotics are generally regarded as the bactericidal agents. the mechanism of action is inhibiting of the enzymes in late stage of peptidoglycan synthesis namely Penicillin binding proteins (PBPs). The inhibitions of PBPs cause morphological changes lead to bacteriolysis and cell death. The relationship among PBPs, morphological changes and bactericidal activities by the beta-lactam antibiotics has been evaluated in this research. Cefpirome, Cefepime, Imipenem and Meropenem were tested against susceptible strain of P. aeruginosa, E. cloacae and E. coli by time kill method. Cefpirome and Cefepime demonstrated bactericidal properties to E. coli from 4 MIC, whereas E. cloacae showed regrowth to both drugs in concentration range from 1/4MIC-128MIC after 24 hours of exposure. For morphological changes both drugs established the filamentous cell on both Enterobacteria, which related to PBP3 binding as primary target of Cephalosporins. Interestingly, Cefepime above 32MIC man

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