Supercritical Fluid Extraction and Characterisation of Urtica Dioica L. Leaves Pigments

TL;DRAbstract

Extraction and fractionation of green and yellow pigments from stinging nettle leaves was studied using ethanol-modified carbon dioxide as a solvent. The extracts were analysed by means of HPLC and RP-HPLC systems. The effect of operation conditions was analysed in a series of experiments at pressures from 20 MPa to 28 MPa, temperatures between 25 °C and 60 °C, and ethanol concentration in CO2 between 0 and 7.4 wt.%. The main result was that the best conditions for the supercritical fluid extraction of pigments were ethanol concentration in CO2 4.5 - 7.4 wt.%, temperature 40°C and pressure 28MPa and that degradation of pigments was eliminated with decrease of temperature of micrometer valve and addition of small amount of antioxidant to ethanol.

Chat with Paper

AI Agents for this Paper