Overcome of grape chemical barriers by the fungal pathogen Botrytis cinerea

TL;DRAbstract

Botrytis cinerea in contact with mature grape berries encounters an environment particularly rich in polyphenols and PR proteins, where the stilbenic phytoalexin trans-resveratrol may accumulate. To mimic conditions similar to those found in grape berries, B. cinerea was grown in vitro with grape PR proteins and polyphenols extracted from mature grapes and with trans-resveratrol. Results showed that in the presence of highly toxic amounts of trans-resveratrol, grape polyphenols allowed total recovery of fungal growth, and proteins allowed partial recovery. These resveratrol-polyphenol or resveratrol-protein combinations also induced a strong release into the medium of laccase activity, which is likely to be involved in trans-resveratrol detoxification. The grape protein pattern changed during fungal growth; most grape proteins quickly disappeared from the culture when polyphenols and trans-resveratrol were present together. Similar protein patterns were obtained in vitro by incubating

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