Establishment of Assay System for Immunoregulatory Factors Using Whole Cell Culture of Mouse Spleno-Cytes

TL;DRAbstract

We established the assay system for immunoregulatory factors using whole cell culture of mouse splenocytes to screen immunoregulatory factors and to clarify the mechanisms by which they regulate immunoglobulin (Ig) production. Adhesive cells such as macrophages and antigen-presenting cells enhanced Ig production of B lymphocytes in the presence of lipopolysaccharides, but the susceptibility to adhesive cells was different with Ig classes. In the absence of adhesive cells, mitogens, such as lipopolysaccharides, pokeweedmitogen and phytohemagglutinin stimulated Ig production, and adhesive cells enhanced the stimulatory effect of mitogens. The effect to mitogens and adhesive cells was different with Ig classes. Milk proteins, such as lactoferrin, β-lactoglobulin, α-casein and β-casein stimulated IgA production, and adhesive cells modified their IgA production stimulating activities. These results indicate that the assay system is useful for screening of immunoregulatory factors and clarif

Chat with Paper

AI Agents for this Paper