Obtención de Rellenos Subdérmicos a partir de Ácido Hialurónico y Fibroblastos en Ratas Wistar

TL;DRAbstract

Title: Obtention of subdermic fillers from fibroblasts and hialuronic acid in Wistar rats. Objetive: To obtain and evaluate a tissue equivalent combining autologous fibroblasts from Wistar rats and hialuronic acid for the development of facial filler in a pilot study. Materials and Methods: Human fibroblasts were obtained and cultured in vitro, cell counts and viability were evaluated using Neubauer chamber and followed for five days. The reproduction rate of the human fibroblasts was determined at different concentrations of hialuronic acid in vitro, through photographic evaluation and images analysis. The subdermic fillers compounded of fibroblasts and hialuronic acid, were implanted in the subcutaneous cellular tissue of four Wistar rats. The in vivo was evaluated through histopathology. Results: .Human fibroblasts duplicated their cell count inside the boxes every 48 hours, at 2 % and 12 % hialuronic acid concentration. The Wistar rats used in this pilot study tolerated the interve

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