Analysis of siderophore production by Rhizobium meliloti 220-5

TL;DRAbstract

A universal chemical assay (Schwyn and Neilands , 1987) was used to detect the production of siderophore in a range of rhizobacterial strains. Siderophore production was found to be strain specific In Rhizobium. The product ion of siderophore by Rhizobium meliloti 220-5 was examined in detail. Using the universal assay to test samples taken during growth of the bacterium in an iron deficient medium, it was established that product ion of the siderophore commenced at the onset of exponent ial growth.
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\n The cell free supernatant of R.meliloti 220-5 grown under iron deficient conditions and shown to contain a siderophore, was tested for the presence of catechol or hydroxamate groups. Neither group was detec ted.The cell free supernatant of R.meliloti 220-5 grown under iron deficient conditions and shown to contain a siderophore, was tested for the presence of catechol or hydroxamate groups. Neither group was detected.The cell free supernatant of R.meliloti 220-5 grown under ir

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