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Open AccessArticle10.1501/vetfak_0000002521

Arthritisli ishalli buzağılarda eklem sıvısı proteinlerinin SDS-PAGE ile elektroforetik analizi

ALTINTAŞ Arif UYSAL-2012-01-01-Ankara Üniversitesi Veteriner Fakültesi Dergisi
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TL;DRAbstract

In the study, synovial fluid samples obtained from clinically healthy calves (control) and diarrheic calves with arthritis were examined by electrophoresis (SDS-PAGE) to determine the distribution of denatured proteins for understanding their clinical importance. In the study, 3-29 days old 7 healthy calves (control) and 19 diarrheaic calves observed in arthritis (2-75 days old) were used as material. Synovial fluid proteins were separated by SDS-PAGE (7.5% + 10% SDS-containing acrylamide, on 8x10 cm mini-gel) according to the molecular weights. Protein bands with the molecular weight of 48-50 kDa and 56-57 kDa were the main bands after SDS-PAGE analysis of joint fluids of calves with and without arthritis. Eighteen protein bands were dominantly found in arthritic group. As a result, joint fluid protein bands with the molecular weights of 205.5; 200-190; 144-140; 110-100; 6462; 40-39 and <10 kDa may be important together or individually to determine the degree of severity of arthritis

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In the study, synovial fluid samples obtained from clinically healthy calves (control) and diarrheic calves with arthritis were examined by electrophoresis (SDS-PAGE) to determine the distribution of denatured proteins for understanding their clinical importance. In the study, 3-29 days old 7 healthy calves (control) and 19 diarrheaic calves observed in arthritis (2-75 days old) were used as material. Synovial fluid proteins were separated by SDS-PAGE (7.5% + 10% SDS-containing acrylamide, on 8x10 cm mini-gel) according to the molecular weights. Protein bands with the molecular weight of 48-50 kDa and 56-57 kDa were the main bands after SDS-PAGE analysis of joint fluids of calves with and without arthritis. Eighteen protein bands were dominantly found in arthritic group. As a result, joint fluid protein bands with the molecular weights of 205.5; 200-190; 144-140; 110-100; 6462; 40-39 and <10 kDa may be important together or individually to determine the degree of severity of arthritis

Keywords

Synovial fluidPolyacrylamide gel electrophoresisArthritisMolecular massPathophysiologyMedicineGel electrophoresisInternal medicine

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