Desenvolvimento de lipossomas catiônicos contendo ácido hialurônico para a veiculação de DNA.

TL;DRAbstract

This research has the goal of develop a vaccine composed of liposome containing DNA and covered by hyaluronic acid as a new strategy for gene delivery. The hyaluronic acid is a natural polysacaride with mucoadhesive properties and has been used in vaccines using nasal route. This biopolymer allows the increase in the gene delivery in liposome systems because it has specific sinals for the cells, CD44 and RHAMM. The liposomes use in this research are composed of egg phosphatidylcholine(EPC), 1,2-dioleoyloxy-3-trimethylammonium propane(DOTAP), l-alpha-dioleoyl phosphatidylethanolamine(DOPE), and hyaluronic acid used was of low molecular weight (6 and 16 kDa). The first step study the influence of the kind of liposome (extruded liposome or DRV -"Dehydrated-rehydrated") and polymer mplecular weight. The final properties of the systems were checked. Extruded liposomes show diameter results more reproductive compared to DRV. The complexe of HA 16 kDa and extruded liposomes has the diameter i

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