Desenvolvimento de processo de recuperação de aprotinina a partir de efluente industrial centrado em cromatografia em quimotripsina imobilizada

TL;DRAbstract

Aprotinin is a pharmaceutical compound used in surgeries and tissue cultures, currently not commercially produced in Brazil.lt is a serine-protease inhibitor present in bovine organs.The objective of this work was to develop a process for the recovery of aprotinin from industrial effluent of bovine insulin production (SPI), based on affinity chromatography onto immobilized chymotrypsin.Three approaches were used: a) chromatography onto immobilized chymotrypsin; b) chromatography onto hydroxyapatite followed by chromatography onto immobilized chymotrypsin; and c) chromatography on immobilized chymotrypsin followed by immobilized metal ion affinity chromatography (IMAC) with cupper as the ligant.Efficiency was analysed based on trypsin and chymotrypsin inhibitions, dot blotting, and electrophoresis assays of the chromatographic fractions.The first approach resulted in aprotinin recovery and purification from the chymotrypsin column with pH step elution gradient, mainly at pH 4.0 and 3.0.

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