Endothelial cell activation during angiogenesis in freely transplanted skeletal muscles in mice and its relationship to the onset of myogenesis.

TL;DRAbstract

The activation time of endothelial cells and myogenic cells (presumptive satellite cells) in small skeletal muscle transplants was examined, as well as in the muscle bed underlying the transplants, using autoradiography and light microscopy. At varying intervals, from 12 to 336 hours (14 days) after transplantation, the transplants and some of the underlying muscle were removed from two or four mice (at each interval), after the injection of each animal with tritiated thymidine 1 hour prior to transplant removal. Thus premitotic cells synthesising DNA were labelled. Only one labelled endothelial cell nucleus was seen in all 20 transplants examined during the first 60 hours. Contrastingly, labelled endothelial nuclei were plentiful in muscles underlying the transplants from 36 hours post-transplantation. There was no evidence of a functional vascular supply in the transplants until 72 hours when one of the eight transplants examined at this time showed very slight peripheral revasculari

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